Isolation of endophytic fungi from Astragalus membranaceus (Fisch.) Bge.and analysis of their secondary metabolites
ZHENG Xue
XU Hui-chao
LI Wei
ZHENG Chun-ying
Abstract：Objective:To investigate the endophytic fungi from Astragalus membranaceus (Fisch.) Bge.and their secondary metabolites in order to find novel resources.Methods:Endophytic fungi were isolated from Astragalus membranaceus (Fisch.) Bge.by using the method of plate cultivation,and the antimicrobial activities of their secondary metabolites were determined by filter paper method.Meanwhile,with formononetin and Astragalus membranaceus (Fisch.) Bge.real samples as reference substances,the secondary metabolites of these endophytic fungi were analyzed by HPLC,and the target endophytic fungi were identified by rDNA ITS sequence homology analysis combined with their physiological and biochemical characteristics.Results:The fermentation broths of 25 strains of endophytic fungi (83.33％ of the total isolated strains) showed different antimicrobial activities,and the HPLC analysis indicated that there were the same corresponding chromatographic peaks appeared in the fermentation broths of HZ13 as its host plants at the same retention times,then the HZ13 strain was identified as Alternaria alternata.Conclusion:Diverse antimicrobial natural products exist in the secondary metabolites of endophytic fungi from Astragalus membranaceus (Fisch.) Bge.,and HZ13 can produce similar or new active metabolites as its host.These findings provided a new idea and approach for using endophytic HZ13 to ferment and produce the components of Astragalus membranaceus (Fisch.) Bge.
Keywords：Astragalus membranaceus (Fisch.) Bge.endophytic fungiantimicrobial activitysecondary metabolites
Publication Date：2017-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：6( 1376-1381 )