Neuroprotective effects of 18β-glycyrrhetinic acid against focal cerebral ischemia reperfusion injury in rats
WANG Zhi-guo
WU Liang-bing
GUAN Lei
PAN Yi
Abstract：Objective:We investigated the neuroprotective effect of 18β-glycyrrhetinic acid (18β-GA)against ischemia-reperfusion (I/R) injury in cerebral neurons in Sprague-Dawley rats.Methods:18β-GA (50,100 or 150 mg·kg-1,i.p.) was administered 1 h after the start of ischemia and 5 h after reperfusion.Neurological deficit scores and infarct volumes were evaluated 24 h later.Neuronal apoptosis was assessed by performing terminal deoxynucleotidyl transferase-mediated dUTP-nick end labeling (TUNEL) staining,and pathological brain damage was estimated by performing hematoxylin and eosin staining.In addition,Caspase-3 protein expression was detected with Western blot.Results:18β-GA significantly reduced the brain infarct area,resolved neurological deficit,reduced the pathological damage,and ameliorated neuronal apoptosis in hippocampal CA1.It also significantly reduced the levels of Caspase-3 protein expression.Conclusion:These results indicated that 18β-GA is neuroprotective and may be useful in reducing the damage caused by stroke.
Keywords：18β-glycyrrhetinic acidcerebral ischemia reperfusion injuryapoptosis
Publication Date：2017-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：7( 1315-1321 )