Establishment of fingerprint analysis method of GBE50 flavonoids
WANG Dan-dan
YAN Yu-jiao
WANG Jun
WEI Ya-fang
ZHANG Xue-yi
WU Pei-ying
Abstract：Objective:To develop an analysis method for establishment of the fingerprint of Ginkgo Biloba Extract 50 (GBE50) and Ginkgo Biloba Tablets based on HPLC-DAD,and obtain the common peaks of 10 batches of GBES0 samples using a UPLC/QTOF-MS analysis method combined with an automated MSE technique.Methods:Agilent Poroshell 120 SB C1s column (4.6 mm × 150 mm,2.7 μm) was used as the separation column.Gradient elution was performed using mobile phase consisting of 0.1％ phosphoric acid and acetonitrile.The flow rate was 1 mL · min-1,detecting wave length was 254 nm and column temperature was 35 ℃.Results:The fingerprint was established.Meanwhile,41 common peaks were identified in the assistance of LC-MS.Conclusion:The fingerprint of GBE50 and Ginkgo Biloba tablets were established to improve the quality standards and provide new methods for quality evaluation.
Keywords：Ginkgo Biloba Extract 50 (GBE50)quantified fingerprintflavonoids
Publication Date：2017-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：6( 1194-1199 )