Simultaneously determination of 7 anthraquinone derivatives in the herb-pair of Radix et Rhizoma with HPLC method
PANG Ting
XIE Zhen
CHEN Yong
LI Yao-hua
WEN Mei-rong
YANG Chun-mei
MAI Lan-yin
DENG Li-hong
Abstract：Objective:To study on the changes of the 7 anthraquinione derivatives in the herb-pairof Radix et Rhizoma,including emodin,rhein,chrysophanol,physcion,aloe-emodin,sennoside A,and sennoside B.Methods:The analysis was achieved with a Dikma Dimonsil-C18 column(250 mm ×4.6 mm,5 μm),with gradientelution of 0.1％ (v/v) phosphoric acid in water and acetonitrile as the mobile phase.The flow rate was 1 mL·min-1,and the detect wavelength was set at 256 nm.Results:All the calibration curves showed good linearity (r≥0.999) in cetain concentration ranges.The RSD of the 7 analytes was all less than 3.0％,and the recovery rates were 94.54％ ～ 103.1％.The 7 anthraquinione derivatives were changed after compatibility.Comparing with single Radix et Rhizoma,Radix et Rhizoma and Persicae Semem,the sennoside B,rhein and physcion in Radix et Rhizoma and Moutan Cortex,Radix et Rhizoma and Kansui Radix were all increased,the sennoside A and emodin in Radix et Rhizoma and Coptidis Rhizoma,Radix et Rhizoma and Aurantii Fructus Immaturus were decreased,especially,the group of Radix et Rhizoma and Coptidis Rhizoma.The chrysophanol in Radix et Rhizoma and Coptidis Rhizoma was decreased significantly and sharply.Conclusion:The contens of anthraquinones and sennosides in Radix et Rhizoma were mostly changed by different compatibilities,which indicated that the mechanism in compatible herbs of Radix et Rhizoma affected the dissolution of anthraquinones,and the effect of the phase to the phase,which may contribute to the characteristics of efficacy of Radix et Rhizoma.
Keywords：HPLCsimultaneously determinationcompatibilitypair herbs of Radix et Rhizomaanthraquinone derivatives
Publication Date：2017-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：10( 1079-1088 )