Protective effects of Saponins from Marsdenia Tenacissima on the CCl4-induced liver fibrosis in male rats
ZHANG Xiao-lei
SU Yuan
FANG Hai-ming
WANG Jia-jia
ZHANG Li-jiu
Abstract：Objective:To study the protective effects and mechanisms of Saponins from Marsdenia Tenacissima (SMT) on the CCl4-induced hepatic fibrosis in male rats.Methods:SD rats were randomly divided into six groups:normal control group,model group,SMT (10,20,40 mg· kg-1) groups and positive control group (colchicine 0.1 mg·kg-1).Hepatic fibrosis was induced by CCl4 twice a week for 12 weeks.From the 4th week,all the therapeutic groups were treated with the SMT and the colchicine,normal and model rats were treated with equal dosage of saline.At the end of the twelfth week,the liver and spleen index were calculated,the levels of HA,LN,CⅣ in serum and Hyp,SOD,MDA,GSH-Px in liver tissues were measured.HE and Masson staining were used to evalulate the degree of hepatic fibrosis.The expression of α-SMA in liver tissues was analyzed by immunohistochemistry,and TGF-β1 and Smad7 were detected by Western blot.Results:Compared with the model group,SMT not only reduced the liver and spleen index,the serum contents of HA,LN,CⅣ and Hyp,MDA in liver tissue,increased SOD and GSH-Px activity,but also improved the morphologic changes of liver fibrosis.Moreover,it obviously decreased the α-SMA,TGF-β1 expression and increased Smad7 expression in the liver tissues.Conclusion:SMT significantly reduces CCl4-induced liver fibrosis in male rats,the mechanism may be due to its antioxidant ability and inhibiting TGF-β1/Smad signal transduction pathway.
Keywords：Saponins from Marsdenia Tenacissimaliver fibrosisantioxidantTGF-β1/smad signaling pathway
Publication Date：2017-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：6( 1047-1052 )