An LC-MS/MS method for determining Zileuton in rat plasma and its pharmacokinetic properties
TANG Qiao-xin
XU Jun-peng
PAN Chao
TANG Ming-hai
WAN Li
Abstract：Objective:To establish an LC-MS/MS method for determining Zileuton in rat plasma and study the pharmacokinetic properties of Zyflo and Zileuton.Methods:Rats recieved Zyflo and Zileuton by intragastrical administration,plasma samples were extracted at different time.Then the concentrations of Zileuton in plasma were determined.Pharmacokinetic parameters were calculated with DAS 2.0 software.Results:Calibration curve of Zileuton was linear in range of 1 ～ 1 000 ng· mL-1 (r =0.999 92),RSD of intra-and inter-day were less than 15％.Extraction recoveries and stability were between 85％ ～115％.t1/2 of Zyflo and Zileuton were 6.091 h and 7.91 h,and the relative bioavailability value was 138.90％.Conclusion:The method is successfully applied for determining Zileuton in rat plasma.Zyflo can be obsorbed better in rat,which can be used for the determination of generic drug study.
Keywords：LC-MS/MSZileutonpharmacokinetics
Publication Date：2017-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：5( 1042-1046 )