Isolation and purification of nobiletin and tangeretin from Pericarpium Citri Reticulatae by multi-solvent partition method
SONG Yu-peng
LIU Kai-yang
CHEN Hai-fang
HU Yuan-xiang
TAN Shu-shu
YANG Wu-liang
Abstract：Objective:To establish an efficient method for the isolation and purification of nobiletin and tangeretin of high purities from Pericarpium Citri Reticulatae.Methods:Multi-solvent partition method was applied to separate nobiletin and tangeretin from the chloroform extract of Pericarpium Citri Reticulatae.The optimized condition of multi-solvent partition of nobiletin and tangeretin obtained by multiple tests was as follows:the extraction solvent was mixture of acetone and light petroleum 6∶5 (v∶ v),the separation liquid was water,and the ratio of extraction solvent to separation liquid was 11∶5 (v∶ v).Results:The purities of nobiletin and tangeretin obtained by this method were not less than 98％ as determined by HPLC peak area normalization method.Conclusion:This study established a simple and efficient method to obtain nobiletin and tangeretin from chloroform extract of Pericarpium Citri Reticulatae.
Keywords：multi-solvent partition methodisolation and purificationPericarpium Citri Reticulataenobiletintangeretin
Publication Date：2017-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：5( 952-956 )