Rapid determination of linderane and norisoboldine in Radix Linderae by near-infrared spectrometry

DU Wei-feng; WU Yao; YUE Xian-ke; LING Jue; GU Chao; GE Wei-hong

Abstract：Objective:To established a near-infrared spectroscopy quantitative model for simultaneous analysis of linderane and norisoboldine in Radix Linderae.Methods:First,the chemical values of linderane and norisoboldine in Radix Linderae were determined by high performance liquid chromatography.Fourier transformation near-infrared spectroscopy (FT-NIR) combined with partial least square (PLS) regression was used to establish and optimize the quantitative calibration model,choosing the spectrum ranges of 4 412 ～4 347 and 8 720 ～ 4 458 cm-1 and " Second derivative + SNV" mode.The calibration model was evaluated by the correlation coefficient (r),the root-mean-square error of calibration (RMSEC),and the root mean square of cross-validation (RMSECV) of the calibration model as well as the root mean square of prediction (RMSEP) of the prediction model.Results:For the quantitative calibration model the r,RMSEC,RMSECV of linderane and norisoboldine were 0.999 9,0.000 673,0.040 11 and 0.990 5,0.027 1,and 0.171 74,respectively.For the prediction model the r and RMSEP of linderane and norisoboldine were 0.917 0,0.009 54 and 0.903 4,0.076 3,respectively.Conclusion:The established near-infrared spectroscopy quantitative model is relatively stable,accurate and reliable for the simultaneous quantitative analysis of linderane and norisoboldine,and is expected to be used for the rapid determination of linderane and norisoboldine in crude drug of Radix Linderae.