An HPLC method for determination of the content of purinostat dihydrochloride bulk drug
XU Jun-peng
PAN Chao
TANG Qiao-xin
WAN Li
Abstract：Objective:To establish a high performance liquid chromatography (HPLC) method for the determination of the content of purinostat dihydrochloride bulk drug.Methods:The determination was performed on a Waters Xbridge C 18 column (150 mm × 4.6 mm,5 μm) with the mobile phase consisting of a mixture of 0.01 mol· L-1 phosphate buffer (pH =3.0) and acetonitrile by gradient elution at a flow rate of 1.0 mL· min-1 The column temperature was 30 ℃,and the detective wavelength was 280 nm.Results:The linear range of the calibration curve for purinostat dihydrochloride was 6.25 ～ 200 μg· mL-1 (R2 =0.999 7,n =6).The average recovery was 100.40％ and RSD was 1.13％ (n =9).Conclusion:The method is specific,accurate,sensitive and suitable for the determination of the content of purinostat dihydrochloride bulk drug.
Keywords：HPLCpurinostat dihydrochlorideassay
Publication Date：2017-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：5( 704-708 )