Comparison of intracellular kinetics of two kinds of PLGA nanoparticles with different zeta sizes
CUI Ya-nan
YANG Xiao-qin
ZHANG Zhi-yun
DONG Hao
SHAN Kun
Abstract：Objective:To compare the difference of intracellular kinetics between PLGA nanoparticles with zeta size of around 100 nm and 150 nm respectively.Methods:PLGA nanoparticles loaded with coumarin-6 was prepared by emulsion solvent evaporation method.Melanoma tumor cells (B16) and glioma cells (U87) were used as cellular uptake models.After incubating with nanoparticles for 0.5,1,4,6 and 17 h,the endocytosed nanoparticles was quantified by detecting the content of coumarin 6 in the cellular lysate by fluorospectrophotometer.The data obtained was analyzed by GraphPad Prism program and the difference was evaluated by using student's t-test.Results:After incubating for 0.5 h,the intracellular quantity of PLGA nanoparticles of 100 nm exhibited significant difference from the particles of 150 nm,and the difference was enlarged as time going.The intracellular rate of nanoparticles of 150 nm decreased obviously by the end of 6 h and after.Conclusion:PLGA nanoparticles of 100 nm intend to be endocytosed more easily and quickly than particles of 150 nm in both U87 cells and B16 cells.And the overall intracellular amount of PLGA nanoparticles of 150 nm is less than that of nanoparticles of 100 nm.
Keywords：PLGA nanoparticlesendocytic kineticscellular uptakezeta sizemelanoma tumor cellsglioma cells
Publication Date：2017-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：6( 698-703 )