Oxymatrine improves fat-induced insulin resistance of ApoE-/-mice by inhibiting inflammatory cytokines
WANG Chao
ZHANG Hui-xin
XING Han-ying
WANG Xing
ZHANG Zhe
Abstract：Objective:To investigate the effect of oxymatrine on hepatic inflammatory cytokines in fatinduced insulin resistance (IR) mice,and investigate the effect on c-Jun NH2-terminal kinase (JNK) pathway.Methods:ApoE-/-mice fed with high fat diet for 16 weeks were selected as IR animal model and randomly divided into the model group,oxymatrine 25,50,100 mg· kg-1 groups.C57BL/6J mice were selected as the normal control group.There were 10 mice in each group.The mice were gavage with oxymatrine for 8 weeks.Glucose tolerance test were conducted.Fasting blood glucose (FBG),cholesterol (TC),triglyceride (TG),fatty acid (FFA) and serum insulin (FINS) were detected.The expression levels of tumor necrosis factor-α (TNF-α),interleukin-6 (IL-6),interleukin-1β (IL-1 β) in hepatic cells were examined by real-time PCR and western-blot.The p-JNK and JNK expression were examined by western-blot.Results:Oxymatrine reduced the levels of FBG,TG,TC,FFA and improved insulin resistance.Compared with the model group,the expression of IL-6 decreased in the oxymatrine 25 mg·kg-1 group.TNF-α,IL-6,IL-1β,p-JNK/JNK expression decreased in the oxymatrine 50,100 mg· kg-1 group.Conclusions:Oxymatrine inhibits inflammatory cytokines in hepatic cells by inhibiting JNK pathway,thus ameliorates fat-induced insulin resistance in mice.
Keywords：oxymatrinehigh fat dietinsulin resistanceinflammatory cytokinesJNK pathway
Publication Date：2017-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：5( 670-674 )