Effects of anhydroicaritin microemulsion formulation on the immunologic bocytopenic purpura mice

WANG Chen; ZHANG Ke; LI Wan; GUO Yong-fei; SUN Yi; ZHAO Xin; YAO Jing-chun; LI Guang-yan; PU Xiao-ping

Abstract：Objective:To investigate effects of anhydroicaritin microemulsion formulation on the immunologic thrombocytopenic purpura (ITP) mice.Methods:Male BALB/c mice were randomly divided into five groups.Constant ITP was induced in four groups by intraperitoneally (ip) injecting guinea pig anti-mouse thrombocyte antiserum with a dose of 100 μL at 1 ∶ 4 dilution every other day,and drug treatment was given every day at the same time.The control group was ip injected with equal volumes of saline.The four groups of the thrombocytopenia mice were treated with microemulsion matrix,rhTPO 3 000 U · kg-1,drug Y003 1.25 mg· kg-1 (Y003-L),and drug Y003 5.0 mg· kg-1 (Y003-H),respectively.The control group was treated with microemulsion matrix.After eleven days of treatment,the blood and spleens were taken and the following indices were determined:complete blood count,organ-to-body weight ratio,multiparameter flow cytometry reticulated platelets counts,TPO content in peripheral blood by ELISA test,and histopathology test of spleen.Results:The platelet counts of peripheral blood of groups Y003-L and Y003-H were significantly increased (P ＜ 0.05).Reticulated platelets counts,organ-to-body weight ratio,and TPO content in the peripheral blood of group Y003-L were significantly decreased (P ＜ 0.05).Conclusion:Oral anhydroicaritin nicroemulsion formulation can improve the symptoms of thrombocytopenia in ITP mice effectively.The mechanism of anhydroicaritin microemulsion's beneficial effect may be related to the C-Mpl receptor-mediated stimulation of proliferation and differentiation of platelets.