Determination of memantine hydrochloride with pre-column derivatization HPLC
LOU Shu-han
WANG Dong
GU Xiao-tong
WANG Yi-fan
LI Xue-ming
Abstract：Objective:To establish a pre-column derivatization HPLC method for determination of memantine hydrochloride.Methods:4 mL 5％ triethylamine acetonitrile solution and 2 mL 5％ benzoyl acetonitrilc solution were added into the drug solution sequentially as deacid and derivatization reagents respectively,and derivatization reaction was conducted through shaking the mixture in water bath(40 ℃) for 10 min.Then acetonitrile was added to the fixed volume to obtain the final sample solution for HPLC determination.The separation and determination were achieved on a SunFireTM C18 column(150 mm × 4.6 mm,5 μm) with the mobile phase of acetonitrile-water (80∶ 20).The flow rate was 1.0 mL·min-1 and the wavelength was set at 254 nm.Results:The sample solution was stable within 12 h and he linear range of the calibration curve was 0.040 00 ～ 0.800 0 mg· mL-1.The average content of 6 samples was 99.21％,and the RSD was 1.78％ (n =6).Conclusion:The method is simple,accurate,reliable,and reproducible.The derivatization reaction is rapid with good stability,which can be used for the determination of memantine hydrochloride.
Keywords：memantine hydrochloridepre-column derivatizationHPLCbenzoyl chloridetriethylamineacetonitrile
Publication Date：2016-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)