Optimization of water extraction and purification process of Lumbricus by multiple indicators grading method
LI Ting
YANG Jun
ZHANG Tong
Abstract：Objective:To optimize the extraction and purification process of Lumbricus.Methods:By using the content of protein and the activity of enzyme measured by Coomassie brilliant blue method and fibrin plate method respectively as indicators,combined with the rate of extractum,the extraction process of Lumbricus was optimized by orthogonal experiment.Hydride generation atomic absorption spectrometry was used to determine the content of arsenic after microwave digesting of samples.Results:The concentration of lumbrokinase showed a good linear relationship with soluble circle area in the range of 2 000 ～ 10 000 U·mL-1.The best extraction process was as follows:soaking Lumbricus coarse powder with 8 times of water for 30 min followed by ultrasonic extraction for 3 times,each time 30 min.The best purification method was high speed centrifugation.Conclusion:The optimized process is stable and feasible,which provides a new choice for making full use of the effective components of Lumbricus.
Keywords：LumbricusCoomassie birlliant blue methodfibrin plate methodarsenic
Publication Date：2016-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)