Genetic polymorphism of GNB3 C825T in association with efficacy of metoptolol

HAN Lu-lu; LI Na; JIANG Xiong-jing; LIU Yu-qing; LIU Hong; LI Yi-shi

Abstract：Objective: To investigate the association of the polymorphism of GNB3 C825T with changes in blood pressure after metoprolol treatment in hypertensive patients. Methods: The hypertensive patients were treated with metoprolol for 8 weeks. The clinical measurements of baseline and after treatment included blood pressure and ambulatory blood pressure. The polymerase chain reaction-restriction fragment length polumorphism (PCR-RFLP) was used to detect the single nucleotide polymorphism (SNP) of GNB3 gene. The ANOVA and stepwise multiple regression analysis were used to assess whether gene polymorphisms are associated with cardiovascular response to metoprolol. Results; Metoprolol produced a significant decrease in the parameters of blood pressure and heart rate (P<0.01). GNB3 C825T genotype was associated with the decreases in nocturnal systolic blood pressure( SBP) and diastolic blood pressure(DBP):CC: (11.1 ±9.3) mmHg/(7. 9 ±5. 8) mmHg; CT: (5. 8 ± 12. 9) mmHg/ (4.5 ±8.6) mmHg; TT:(3. 6 ± 13.7) mmHg/(3.0 ±7.9) mmHg. The falls were significantly different between the genotypes (CC > CT >TT, P <0. 05) , which was further confirmed by stepwise multiple regression analysis. Conclusion; CNB3 C825T polymorphism is associated with the decrease in nocturnal blood pressure response to metoprolol.