Immunogenicity and lone-term hematological toxicity of Ⅲ valence rotavirus gene reassortment vaccine
HAN Jian
CAI Yong-qing
WU Yong-jie
ZHOU Xu
WEI Hu-lai
GAO Ming-tang
JING Tao
Abstract：Objective:To evaluate the immunogenicity and long-term hematological toxicity of Ⅲ valence rotavirus gene reassortment vaccine in Sprague-Dawley(SD)rats.Methods:Rats(n=30)were repeatedly ig immunized with Ⅲ valence rotavirus gene reassortment vaccine at 2 mL for 3 times.Three weeks after the first immunization,and 1 and 4 weeks after the third immunization,10 rats were respectively sacrificed for examination of the specific antibody against vaccine,hematology and bone marrow histopathology.Results:The antibody titers were significantly higher in vaccine group than control group at each detected point.The antibody against G1,G2,G3,G4 and G10 were found three weeks after the first immunization.and then increased gradually.The antibody titers of G2,G3 and G4 were significantly higher than G1 and G10(P＜0.01 or P＜0.05).The lymphocyte count and mean corpuscular hemoglobin(MCH)in vaccine group showed reversible changes,but did not show apparent toxicity.There were no apparent changes in bone marrow histopathology.Conclusion:Ⅲ valence rotavirus gene reassortment vaccine has immunogenicity against rats and has no apparent bone marrow toxicity in rats.
Keywords：Ⅲ valence rotavirus gene reassortment vaccineimmunogenicityhaematological systemlong-term toxicity
Publication Date：2010-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)