Preparation and quality control of neogambogic acid hydroxypropyl-β-cyclodextrin inclusion complex
Abstract：Objective: To study the preparation, identification and quality control of neogambogic acid hydroxypropyl-β-cyclodextrin inclusion complex (neogambogic acid-HP-β-CD). Methods:The inclusion complex was prepared by freeze-drying technique and was identified by differential scanning calorimetry (DSC), fourier transformation-infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and phase solubility methods, respectively. A HPLC method was established to determinate the content of neogambogic acid in the inclusion complex. Results: The identification suggested the formation of inclusion complex of 1:1 mol ratio with stability constant K(_a (117 L·mol~(-1)). The content determination method showed a linear range of 4.12～24.72 μg·mL~(-1)(r=0.999 9) and a mean recovery rate of 100.1% with a RSD of 0.48%. Conclusion:Freeze-drying method is simple and ad-vantageous to prepare inclusion complex and it can increase the solubility of neogambogic acid.
Keywords：neogambogic acidhydrresuoxypropyl-β-cyclodextrininclusion complexfreeze-drying tech-niquehigh performance liquid chromatography (HPLC)
Publication Date：2010-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)