Effect of Deer bone and Seeds peptide capsule on osteoblasts proliferation, toxicology and stability
Abstract：Objective: To evaluate the anti-osteoporosis effect of Deer bone and Seeds peptide (DBS) cap-sule. Methods:In vitro anti-osteoporosis effect was evaluated by MTT reduction assay, and by detection of alkaline phosphatase (ALK) and mineralized nodes in osteoblasts from SD rats. The accelerated stability tests included de-terminations of appearance, microorganisms, heavy metals, disintegration time, and the contents of water, ash and DBS. Results: DBS capsule enhanced cell proliferation, ALK activity and the number of mineralized nodes in rat osteoblasts. Toxicological assessments showed that DBS capsule was completely not poisonous and had no potential of mutagenesis. The content of peptide was≥8.0 g per 100 g in the capsule. The capsules' shelf life reached 24 months. All the indexes were conformed to match GB and the corporation quality standards, including those in mi-croorganisms, heavy metals, disintegration, moisture barrier and ash. Conclusion: DBS capsule is an effective and safe product with controllable quality for enhancing bone mineral density, prevention and control of osteoporosis in middle-aged and senile people.
Keywords：Deer bone and Seeds peptide (DBS) capsuleosteoporosisosteoblasts proliferationtoxicol-ogystability
Publication Date：2010-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)