Separation, purification and structure identification of the antitumor antibiotic component from fermentation broth of a new micromonospora C-3509
Abstract：Objective: To identify the antitumor component extracted from the fermentation broth of a new micromonospora C-3509. Methods: Many kinds of chromatography were used to separate and purify the antitumor component. Then, the identification of the active antitumor component was performed by ultraviolet (UV) scan-ning, high-resolution electrospray ionization mass spectrometry (HR-ESI-MS) and proton nuclear magnetic reso-nance spectorscopy (~1H-NMR). Finally, its antitumor effect was analyzed by DNA damage. Results: The antitu-mor component C-3509B was obtained after the fermentation, detection, isolation and purification of C-3509 strain. Furthermore, the preliminary results of spectral analysis and structural identification for the component C-3509B showed that the structural characteristic of the component was consistent with antitumor antibiotic calicheamicin γ_1~I. Conclusion: C-3509B may be the calicheamicinγ_1~I, a kind of 10-membered cyclic enediynes.
Keywords：micromonospora C-3509enediyneantitumor antibioticcalicheamicin
Publication Date：2010-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)