Determination of plasma aglycon of icariin in Beagle dogs by liquid chromatography-tandem mass spectrometry
MENG Fan-hua
GUO Ji-fen
LI Quan-sheng
SI Duan-yun
ZHAO Yi-min
Abstract：Objective: To establish a specific and sensitive LC-MS/MS method for the determination of plasma aglycon of icariin in Beagle dogs. Methods: Aglyeon of icariin and insternal standard triein were extracted by ethyl acetate from plasma, then separated on a Zorbax C_8 column. The mobile phase consisted of acetonitrile and 5% acetic acid(70: 30). The protonated analytes generated in the positive ion mode were monitored through multiple reaction monitoring(MRM) in an electrospray ionization source. Results: The linear concentration ranges of the calibration curve for aglycon of icariin was 2.5 ～ 250 ng·mL~(-1). The lower limit of quantitation was 2.5 ng·mL~(-1).The inter-day and intra-day precision values was below 13.3%, and the accuracy(relative error) was within ± 5.5%.Conclusion : The method is suitable for the pharmacokinetic studies of aglycon of icariin with the advantages of high sensitivity and specificity.
Keywords：LC-MS/MSaglycon of icariinpharmacokinetics
Publication Date：2010-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：4( 537-540 )