Effect of Glp-1 on lipid metabolism-related genes in insulin-resistant myocytes

HUANG Li-song; WANG Li-qiu; LIN Zhu-sen; JIANG Ming-hong

Abstract：Objective: To study the effect of Glp-1 on insulin sensitivity and the lipid metabolism-related genes in insulin-resistant myocyte model induced by palmitate (PA). Methods: Insulin resistance was established by PA in C2C12 myocytes. To estimate the effect of Glp-1, glucose consumption and glucose uptake were assayed in the insulin-resistant myocytes after Glp-1 treatment for 48 h. In addition, the expression levels of lipid metabolism-related genes were measured by real time PCR method. Results: When C2C12 myocyts were treated for 24 h with 0.25 mmol·L~(-1) PA, the glucose consumption and glucose uptake markedly decreased and the lipid content obviously increased, indicating the resistance to insulin. Glp-1 treatment significantly ameliorated the decrease in glucose consumption and glucose uptake, and remarkably decreased the lipid content as compared with control group. The expression of Fas gene, one of the key genes in the signal pathway of fatty acid synthesis, significantly increased in insulin-resistant myocytes, and Glp-1 treatment inhibited this increase. Several key genes related to fatty acid oxygenolysis did not show the similar changes in the two groups. Conclusion: Glp-1 can increase insulin sensitivity and inhibit the fat accumulation in the insulin-resistant myoeyts induced by PA. Its mechanism may be associated with the regulation of lipid metabolism gene expressions.