Effect of saponins of Rhizoma Polygonati on the behaviors and the hippocampal and cortical expressions of BDNF and TrkB in rats with chronic stress-induced depression
ZHU Ling-li
XU Wei-ping
WEI Wei
XU Ting-juan
Abstract：Objective: To explore the anti-depressive effect of saponins of Rhizoma Polygonati(SRP) and the mechanism, we determined the behaviors and the expressions of brain-derived neurotrophic factor(BDNF) and tyrosine kinase receptor(TrkB) in the hippocampus and cerebral cortex of rata with chronic stress-induced depression. Methods: Forty-eight SD rats were divided into six groups: normal control, depression model, fluoxetine (2 mg·kg~(-1)), SRP(30,60,120 mg·kg~(-1)) groups. Rats were exposed to alternate stressors for 21 days to induce depression. Behavioral indexes and changes in learning and memory were observed before, during and after the experiments. The expression level of BDNF and TrkB in the hippoeampus and cerebral cortex was determined by immunohistochemistry. Results: After chronic stress stimulation, the behavioral indexes showed an obvious depressive state in rats of depression control group as compared with normal control rats. BDNF and TrkB positive cells were observed in each section of the hippocampus and cerebral cortex. The numbers of positive cells and their gray scales were less in the depression control group than in the normal control and SRP(30 ,60 ,120 mg·kg~(-1)) groups. Conclusion: SRP effectively attenuates BDNF and TrkB down-regulation in neurons of the hippocampus and cerebral cortex in rats with chronic stress-induced depression, which may be associated with its anti-depressive effect.
Keywords：depressionsaponins of Rhizoma Polygonatibrain-derived neurotrophie factortyrosine kinase receptor
Publication Date：2010-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：9( 517-525 )