Neuroprotective effect of intranasal administration of nerve growth factor in the mouse MPTP model of Parkinson's disease
ZHANG Hui-nan
PU Xiao-ping
Abstract：Objective: To investigate the neuroprotective effect of intranasal administration of nerve growth factor (NGF) against 1-methyl-4-phenyl-1,2, 3,6-tetrahydropyridine (MPTP)-induced dopaminergic toxicity. Methods: The behaviors of C57 mice were assessed using the spontaneous movement and rotarod tests. DA levels in the striatum were measured using HPLC-EC. We also determined the activities of SOD and GSH-S in the stria-rum. Dopaminergic neurons in the substantia nigra were observed by immunohistochemical staining with anti-tyrox-ine hydroxylase (TH) antibody. Results: When intranasally administered before MPTP, NGF reduced behavioral deficits, increased the levels of dopamine and dopamine metabolites in the striatum, and increased striatal SOD and GSH-S activity as compared to mice treated with MPTP alone. Immunohistochemical study with anti-tyrosine hydroxylase antibody showed that intranasal administration of NGF had a protective effect against MPTP-induced neuronal damage in the substantia nigra of mice. Conclusion: When intranasally administered before MPTP, NGF may be able to protect C57 mice against MPTP-induced neuronal damage.
Keywords：nerve growth factor (NGF)1-methyl-4-phenyl-1236-tetrahydropyridine (MPTP)Parkinson's disease modelintranasal administration
Publication Date：2010-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：5( 152-156 )