Inhibition of phosphodiesterase activity by chemical constituents of Xylosma controversum Clos and Scolopia chinensis(Lour.) Clos
LING Si-kai
XU Zheng-ren
TU Peng-fei
PU Xiao-ping
Abstract：Objective: To investigate whether the chemical constituents of Xylosma controversum Clos and Scolopia chincnsis(Lour.) Clos have inhibitory effects on phosphodiesterase(PDE) activity. Methods: The test samples was pre-incubated with the enzyme for 30 min at 37 ℃, and the enzyme activity was then measured spec-trophotometrically on a microtiter-plate reader (BioRad 550, USA) by monitoring the rate(change in OD/min) of release of 4-nitrophenol from bis(4-nitrophenyl) phosphate at 405 nm. Results: Of 27 compounds, 3 showed inhi-bition of snake venom PDE activity. They are catechin-5-O-β-D-glucopyranoside, (±)-catechin and xylosmin. Compared with the positive controls EDTA-2Na and cysteine, compounds catechin-5-O-β-D-glucopyranoside, (±)-eatechin and xylosmin had moderate inhibitory effects on snake venom PDE activity, among which (±)-catechin had the strongest effect. Conclusion: The chemical constituents, eatechin-5-O-β-D-glucopyranoside and (±)-cat-echin from Xylosma controversum Clos and xylosmin from Scolopia chinensis (Lour.) Clos, have inhibitory effects on snake venom PDE activity.
Keywords：Xylosma controversum ClosScolopia chinensis (Lour.) Closchemical constituentsphos-phodiesteraseinhibition
Publication Date：2010-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：5( 147-151 )