Preparation of antisense oligodeoxynucleotides-loaded cationic proliposomes and its in vitro characters
LIU Yang
ZHANG Zhen-zhong
SHAO Yan-jiang
LIU Yu-fan
Abstract：Objective:To prepare stable antisense oligodeoxynucleotides-loaded cationic proliposomes. Methods: The cationic liposomes were prepared by film scattering method. Antisense oligodeoxynucleotides were loaded on the particles by adsorption and the proliposomes were obtained by lyophilization. The morphology was investigated by transmission electronic microscope. The size and surface charge of the liposomes were determined by laser particle analyzer. The dissociated oligodeoxynucleotides were released from the liposomes by sephadex column and the entrapment efficiency was determined by an ultraviolet photometer. Results: Trehalose mannitol and glycine were suitable for lyophilization, especially trehalose. The resulting liposomes were global microcapsule in a narrow particle size with a mean diameter of 175 am and 320 am before and after lyophilization. A high Zeta potentials of +32mV and +40mV before and after lyophilization and a high-oligodeoxynucleotides-loading efficiency of 87.6% and 83.21%before and after lyophilization were found. Conclusion: With trehalose as lyoprotechant, the film scattering-lyophilization method can be used to prepare stable cationic liposome proliposomes which appeared to be a promising vehicle for antisense oligodeoxynucleotides.
Keywords：cationic liposome prosomasantisense oligodeoxynucleotideslyophilizationlyoprotectant
Publication Date：2010-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：5( 72-75,86 )