Effect of recombinant human endostain on airway remodeling in murine model of asthma
JIANG Ping
ZHENG Hong
DAI Si-hong
Abstract：Objective: To investigate the effect of recombinant human endostain on airway remodeling in comparison with montelukast and budesonide in a murine model of asthma. Methods: BALB/c mice were randomly divided into five groups: control (n = 10) , asthmatic model (n = 10) ,endostain (n = 10,ip injection of 2.25 mg· kg~(-1) ) , montelukast (n = 10, intragastric lavage of 3 mg·kg~(-1)) and budesonide (n = 10, nebulization of 1 mg ·kg~(-1) )groups. The asthma was induced by OVA sensitization and challenge. VEGF levels in BALF were measured by enzyme 1 inked-immunosorbent assay (ELISA). The airway wall thickness (S1/P1 ) ,the bronchial smooth muscle thickness (S2/P1 ) and the vascular smooth muscle (S3/P2) were measured using an image analysis system. Results: The values of S1/P1, S2/P1 and S3/P2 were (16.4 ± 1.5) ,(2.2 ±0.4) and (5.2 ±1.0) μm·μm~(-1) in control mice, and (35.3 ±4.4),(12.9 ±2.4) and (23. 6 ± 1.2) μm~2·μm~(-1) in asthmatic mice; the values were significantly higher in asthmatic mice than in control mice (P < 0. 05). The values of S1/P1 , S2/P1 and S3/P2 were (24. 5±0. 7) , (5. 5 ±0. 5) and (9.3±3.0) μm~2·μm~(-1) in endostain group; (22. 8 ±4.3), (5.3 ±1.8) and (15.0 ±6.4) μm~2· μm~(-1) in montelukast group; and (21. 4 ± 2. 5 ) , (4. 9 ± 1. 1) and (13.8 ±2.3) μm~2·μm~(-1) in budesonide group. The values were significantly lower in endostain, montelukast and budesonide groups than in asthmatic group (P < 0. 05) , but had no significant difference among endostain, montelukast and budesonide groups (P>0.05). The levels of VEGF were (19.23 ±2.4) pg·mL~(-1) ± in control group, (108. 43±2.0) pg·mL~(-1) in asthmatic group, (70. 26 ±0. 5) pg·mL~(-1) in endostain group, (89. 34±9. 5) pg·mL~(-1) in montelukast group, and (82.33±5.0) pg·mL~(-1) in budesonide group. The levels in asthmatic group were significantly higher than those in endostain, montelukast and budesonide groups (P <0.05). Conclusion: VEGF is over-expressed in asthmatic mice. Recombinnt human endostain as well as montelukast and budesonide attenuate airway remodeling and inhibit the over-express of VEGF.
Keywords：recombiant human endostainairway remodelingasthma model
Publication Date：2009-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：5( 2349-2352,2359 )