Effects of MC004 on fertility of male rats

LUO Yong-wei; SHI Chang; WU Chun-qi; WANG Quan-jun; ZHANG Yu-jie; LIAO Ming-yang

doi:10.3321/j.issn:1003-3734.2009.23.006

Abstract：Objective：To study the toxic effects of MC004 on fertility of male rats. Methods:Healthy and mature Wistar rats (n = 48) were randomly divided into four groups (12 rats in each group). The rats in control group were intravenously injected with normal saline, and the rats in other groups were administered with MC004 at doses of 0.25,0. 50 and 0. 75 mg·kg~(-1) .respectively. All rats were injected once every two days. All the 48 rats were sacrificed after 28 days. The clinical symptoms,body weight,reproductive ability and the pregnancy index were evaluated. Results; At the dose of 0.75 mg·kg~(-1),the fertility index and the pregnancy index were significantly de-creased. There was no difference between the control dams and the dams mated with MC004-treated males,in the mating indices,the mean number of corpus lutea,and the mean number of absorption fetuses. However,there was a significant decrease in the weight of uterus, placenta, ovary, the mean number of implants and the mean number of alive fetuses, and an increase in the mean percentage of preimplantation and the mean percentage of postimplanta-tion as compared with the control group. Conclusion: MC004 has obvious reproductive toxicities to puberty male rats,which can decrease the fertility of puberty male rats 28 days after injection of 0. 75 mg· kg~(-1) ,once every two days.