Simultaneous determination of two ingredients in compound terbinafine cream by reversed-phase HPLC
GUO Min
LI Sa-jing
LIN Cui-hua
ZHANG Zhi-qin
HAN Guo-zhu
Abstract：Objective:To develop an HPLC method for simultaneous determination of two ingredients in compound terbinafine cream. Methods: The chromatographic separation was achieved on a Kromasil-C_(18) column (150 mm×4.6 mm, 5 μm)using a mobile phase consisting of MeOH-NH_4Ac buffer [(1.15 g ammonium acetate
+ 300 mL water + 1 mL glacial HAc adjusted to pH 5～6 with (C_2H_5)_3N) ] (70:30) running at a gradient flow ate : 1.0 mL·min~(-1) at 1～8 min, and then changed to 1.5 mL·min~(-1) at 25 min. The detection wavelength was set at 254 nm. Results: Terbinafine was well separated from triamcinolone (R > 2.0); there was no interference from matrix. The calibration curve showed good linearity over the range of 100～1 600 μg·mL~(-1) for terbinafine (r = 0.999 9) and 10～160 μg·mL~(-1) for triamcinolone (r = 0.999 9). The recovery was 98.7 %～100% for terbinafine and 98.2%～99.8% for triamcinolone. Conclusion: The present method has high specificity and precision, and is
able to simultaneously determine contents of two ingredients in the cream with procedure being simple and, there-fore, can be used for the quality control of the cream preparation.
Keywords：HPLCterbinafinetriamcinolonecream
Publication Date：2009-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：4( 2167-2169,2171 )