A rapid and sensitive HPLC method for determination of bevantolol in human plasma
HAN Yang
TAN Zhi-rong
OUYANG Dong-sheng
GUO Dong
CHEN Yao
PENG Liang
ZHOU Hong-hao
Abstract：Objective: To establish a HPLC method for determination of bevantolol in human plasma. Meth-ods: Liquid-liquid extraction was adopted after the addition of bevantolol and propranolol (an internal standard) in-to the blank plasma. The analysis involved a Plastisil ODS (250 mm×4.6 mm, 5 μm) column. The mobile phase
consisted of acetonitrile:10 mmol methanoic acid amine water solution (including 0.2% formic acid) (35: 65); the flow rate was 1 mL·min~(-1). The detect wavelength was 280 nm, and the column temperature was set at 40 ℃. Results:The peak area of bevantolol in plasma showed good linearity within the range of 15.5～1 984 ng·mL~(-1), and r~2 = 0.999 9. The LLOQ of bevantolol in plasma was 15.5 ng·mL~(-1). The extracted recovery was > 86% ;the relative recovery was 94%～98% ; the intra-and inter-day RSD were < 15%. Conclusion:This method is sensitive, simple and accurate;it can be used for determination of bevantolol plasma concentration and its pharmacokinetic
and bioequivalence study.
Keywords：HPLCbevantololdrug plasma concentration
Publication Date：2009-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：4( 2160-2162,2182 )