Effect of hydroxyurea on apoptosis-related genes and proteins in rat germ cells
ZHOU Li
WU Chun-qi
YANG Bao-hua
WANG Qing-xiu
WANG Quan-jun
YANG Dong-hua
LIAO Ming-yang
Abstract：Objective: To determine the expression levels of apoptosis-related gene mRNA and protein in rat germ cells after hydroxyurea (HU) treatment. Methods: Adult male Wistar rats were injected (ip) with a sin-gle dose of HU (400 mg·kg~(-1)). The total RNA and protein were separated from the testis 6, 12, or 24 h after HU treatment. Results: RT-PCR and Western blotting were conducted for analysis of apoptosis-related gene and the pro-tein products. The expression level of Bax did not change, but that of Bcl-2 significantly decreased at 6 or 12 h after
HU treatment. In contrast, the expression levels of Fas and FasL significantly increased at 12 h. Conclusion: HU in-duces apoptosis of rat testicular germ cells, in which Bcl-2 protein and the Fas signaling system may be involved.
Keywords：ratsmalereproductive toxicityapoptosis
Publication Date：2009-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：5( 2113-2117 )