Determination of azithromycin in human plasma by liquid chromatography-tandem mass spectrometry and its pharmacokinetic study
LIU Qian
SHI Zhen-peng
SHANG Hong-wei
WANG Lu
YANG Lun
WANG Gui-feng
Abstract：Objective; To establish an HPLC-MS/MS method for determining azithromycin in human plas-ma and to study the pharmacokinetics of azithromycin dispersible table in healthy volunteers. Methods; The plasma concentration of azithromycin was determined by HPLG-MS/MS assay, and its pharmacokinetic parameters were calculated by DAS program. Results; The linear regressive curves were obtained in the range of 1. 0 ～ 1 000 ng· mL~(-1) , and recoveries of azithromycin were 88. 7% ～ 113. 2% using this method. Within-day and between-day RS-Ds were less than 15% after a single oral dose of 500 mg azithromycin dispersible tables in 20 healthy volunteers. The pharmacokinetic process fitted with the two compartment open models. The main pharmacokinetic parameters were: t_(1/2)α(5. 174 ±9.0) h; t_(1/2)β(31.4 ±22.7) h; K_e (0.022 ±0.008) h~(-1); T_(max)(1.70 ±0. 66) h; C_(max)(549.8± 242.9) ng·mL~(-1); k_(12)(0. 199 ±0.262) h~(-1); k_(21) (0.217 ±0.463) h~(-1); AUC_(0～96)(3 193 ±1 166) ng·h·L~(-1); AUC_(0～∞)(3 519 ± 1 333) ng·h·L~(-1), respectively. Conclusion:The HPLC-MS/MS method established in this study is accurate and sensitive with no endogenous interference, which can be adapted to pharmacokinetic study.
Keywords：HPLC-MS/MSazithromycin dispersible tablepharmacokinetics
Publication Date：2009-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：3( 1986-1988 )