Experimental study of cytotoxicity of As2O3 alone or in combination with daunorubicin,etoposide,and harringtonineto multidrug resistant leukemic cell
ZHUO Jia-cai
WANG Ming-chun
LIU Huan-xun
LI Ming
NIE Li-ping
DU Xin
CHEN Jun-xiong
Abstract：Objective:To explore the toxicity of As2O3 alone or in combination with daunorubicin (DNR), etoposide (VP16),or harringtanine (H) to sensitive leukamic cell line (K562) or to multidrug resistant (MDR) leukamic cell line (K562/A02).Methods:The cytotoxicity was detected by MTT assay,the combined toxicity of two drugs was analyzed by Chou-Talalay combination index (CI);and the concentration of P-glycoprotein on cell surface and of DNR inside cells were detected by flow cytimetry.Results:The IC50 of As2O3 to K562 and K562/A02 was (2.1±0.2)μmol*L-1 and (1.5±0.3)μmol*L-1 respectively (P>0.05).The CI of As2O3 in combination with DNR,VP16,and H to K562 cell line was 1.73,3.26,and 1.33 respectively,and the corresponding data to K562/A02 was 1.26,1.80,and 1.16 respectively.The fluorescent intensity of P-glycoprotein on cell surface or the fluorescent intensity of DNR inside cell has no significant difference before and after K562/A02 cell line exposed to As2O3.Conclusion:The cytotoxicity of As2O3 to K562 cell line is similar to that of K562/A02 cell line.As2O3 in combination with DNR,VP16,and H has antigonist effect to sensitive K562 and K562/A02 cell line and do not affect P-gp synthesis or P-gp function.
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Publication Date：2001-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：3( 270-272 )