Antiarrhythmic effects of propofol on reperfusion induced ventricular arrhythmia in isolated rat hearts

TIAN Ming; CAO Hong; DUAN Shi-Ming; ZHU Li-yan; ZENG Yin-ming

doi:10.3321/j.issn:1003-3734.2001.02.006

Abstract：Objective:To observe the effect of propofol on ventricul ar arrhythmia in isolated heart of rats induced by reperfusion.Methods :Arrhythmic model in isolated heart of SD rats was created by Lengendorff reperf usion using a solution contaning NaCl 118,KCl 4.7,KH2PO4 1.2,MgSO4 1.2, CaCl2 2.5,NaHCO3 25,glucose 11.1,and EDTA 0.125mmol/L.Forty-seven isolate d heart models were divided into control (Cntrl,n=12),1μg propofol(P1,n =7),3μg propofol(P3,n=9),10 ng verapamil(Ver,n=7),1μg propofol plus 10 ng verapamil（P1+V,n=6),and 3μg propofol plus 10 ng verapamil(P3+V，n= 6) groups.All of the left anterior descending coronary artery was clamped 10 min to result in regional ischemia and reperfused 5 min to create reperfusion arrhy thmia. During reperfusion time, the incidence of ventricular fibrillation (VF) a nd ventricular tachycardia (VT) as well as the duration of VF and normal sinus r hythm (NSR) was measured.Results:The incidence of VF and VT during reperfusion time is 9/12 and 8/12 in Cntrl group;4/7 and 5/7 in P1; 0/9 (P< 0.01 vs. Cntrl) and 2/9 in P3;0/7(P<0.01) and 5/7 in Ver; 0/6(P<0.01) an d 1/6 in both P1+V and P3+V.The duration of VF in group Cntrl and P1 is(143 ±126)s and(146±146)s respectively.In P3,Ver,P1+V and P3+V groups,all the durat ion of VF is(0±0)s(P<0.01).The duration of NSR in group Cntrl and P1 is (106±135)s and(144±149)s respectively,in group P3,Ver,P1+V and P3+V is(262±99 )s(P<0.01),(272±26)s(P<0.05),(270±74)s(P<0.05) and (295±14)s(P <0.01) respectively.Conclusion:As compared with the control group, propofol 3 μg/ml similar to verepamil 10 ng/ml, significantly reduced the incid ence and duration of reperfusion-induced VF.Furthermore,it markedly increased t he duration of NSR in the reperfusion time.Nevertheless, propofol 1 μg/ml prese nted little antiarrhythmic effects.