Study on the regulation effect of cysteine-rich transmembrane bone morphogenetic protein regulator 1-bone morphogenetic protein 4 on ventricular myocyte hypertrophy induced by angiotensin Ⅱ
LI Yu
YANG Long
HE Jiong-hong
OU Qiao-qiao
YANG Ying
Abstract：Objective To investigate whether cysteine rich transmembrane osteogenic protein regulatory factor 1(Crim1)regulates angiotensin Ⅱ(Ang Ⅱ)-induced ventricular myocyte hypertrophy by inhibiting bone morphogenetic protein 4(BMP4).Methods The ventricular myocytes from 1-day-old SD rats were isolated and divided into nine groups according to different intervention methods:Ad-Null(adenovirus empty vector)group,Ad-Null+Ang Ⅱ group,Ad-Null+Ang Ⅱ+Nog(recombinant human noggin)group,Ad-Crim1(recombinant adenovirus vector of Crim1 gene)group,Ad-Crim1+Ang Ⅱ group,Ad-Crim1+Ang Ⅱ+Nog group,Ad-shCrim1(shRNA interference mediated silencing of Crim1 gene recombination adenovirus vector)group,Ad-shCrim1+Ang Ⅱ group and Ad-shCrim1+Ang Ⅱ+Nog group.The mRNA expression of myosin heavy chain beta(β-MHC)and BMP4 was detected by RT-qPCR.The protein level of BMP4 in the whole-cell extracts was determined by Western blot analysis.The cells stained with crystal violet were measured for cell surface area using Image J software.Results AngⅡ intervention significantly increased β-MHC mRNA expression,BMP4 mRNA/protein expression and cell area of ventricular myocytes;the above effects of AngⅡ intervention were significantly inhibited by pre-using of Ad-Crim 1 and Nog.Crim1 gene of ventricular myocytes silenced by Ad-shCriml intervention leading to the similar effects of Ang Ⅱ intervention on β-MHC mRNA expression,BMP4 mRNA/protein expression and cell area,those were significantly inhibited by Nog intervention.Conclusions Crim1 inhibits Ang Ⅱ-induced hypertrophy of neonatal rat ventricular myocytes by decreasing BMP4 expression.
Keywords：Myocardial hypertrophyCysteine rich transmembrane osteogenic protein regulatory factor 1Bone morphogenetic protein 4Angiotensin Ⅱ
Publication Date：2024-12-10
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1125-1130 )