Expression of REEP5 in Kawasaki disease(KD)and its effect on early apoptosis of vascular endothelial cells by affecting mitochondrial function

LI Xiao-ya; LU Qin-mei; LI Cong-zhe

doi:10.3969/j.issn.1672-5301.2024.05.006

Abstract：Objective To investigate the expression of REEP5 in pediatric patients with Kawasaki disease and its effect on early apoptosis of vascular endothelium by affecting mitochondrial function.Methods A total of sixty nine pediatric patients diagnosed with KD from Tangshan Central Hospital between January 2020 and June 2023 were selected as study subjects.They were categorized into two groups:the coronary artery injury group(thirty three cases)and non-coronary artery injury group(thirty six cases).An additional thirty healthy children were selected as the control group.Enzyme immunosorbent assay was used to detect the expression of serum REEP5,apoptosis,angiogenesis,mitochondrial function,cell proliferation and protein expression.Results Compared with the healthy control group,REEP5 was found to be significantly lower in both the coronary artery injury group and the non-coronary artery injury group[(1.05±0.63)vs.(4.64±1.18),(3.16±0.85)vs.(4.64±1.18),P<0.05],and REEP5 in the coronary artery injury group was lower than the non-coronary artery injury group(P<0.05).Platelets,erythrocyte sedimentation rate,leukocytes,prothrombin time,activated partial thromboplastin time,fibrinogen degradation products,fibrinogen were negatively correlated with serum REEP5(P<0.05).In vitro cell experiments,the expression of REEP5 miRNA in the model group was lower than that in the control group[(1.33±0.15)vs.(0.34±0.05),(1.24±0.11)vs.(0.23±0.06),P<0.05]and the number and density of blood vessels in the model group were also lower(P<0.05).Compared with the control group,the cell viability and cell proliferation ability of the blank group were significantly reduced;the LDH activity increased;the mitochondrial membrane potential decreased;the mPTP openness increased(all P<0.05)and the early apoptosis of cells increased(P<0.05).Compared with the blank group,the si-REEP5 group had significantly lower cell viability and cell proliferation ability,a greater increase in LDH activity,a pronounced decrease in mitochondrial membrane potential,a marked increase in mPTP openness(all P<0.05)and a substantial increase in early apoptosis(P<0.05).Compared with the control group,blank group and si-REEP5 group,the REEP5 overexpression group had significantly increased cell viability and cell proliferation ability,a marked decreased LDH activity,notably increased mitochondrial membrane potential,a considerable decreased mPTP openness(all P<0.05)and early apoptosis(P<0.05).Conclusion In pediatric patients with Kawasaki disease,REEP5 is significantly underexpressed,and REEP5 could affect early apoptosis by affecting mitochondrial membrane potential and mPTP openness.