The role and mechanism of neuraminidase 1 in lipopolysaccharide-induced myocardial injury

WANG Xiu-kun; CHEN Xiao-li; YANG Bo

doi:10.3969/j.issn.1672-5301.2024.03.017

Abstract：Objective To investigate the mechanism of neuraminidase 1(NEU1)on lipopolysaccharide(LPS)-induced myocardial injury in rats.Methods From June to July 2023,24 male Sprague-Dawley rats aged 6-7 weeks were raised in the animal room of Wuhan Third Hospital.The rats were purchased from the experimental center of Hubei Provincial Center for Disease Control and Prevention.Firstly,taking NUE1 inhibitor OSE(20 mg/kg)or equivalent amount of saline by continuous oral pretreatment for 3 d,after that,LPS(10 mg/kg)or an equivalent amount of saline was injected intraperitoneally,and the heart was examined by ex vivo perfusion after 12 h.Serum was taken and ELISA was performed to detect the markers of myocardial injury,cTnT and CK-MB.The heart was taken,and the expression levels of Bcl-2 and Bax proteins in myocardial tissues were detected by western.Results Echocardiographic results showed that the cardiac ejection fraction and shortening fraction were significantly reduced in the LPS group,while the cardiac function was improved in the LPS+OSE group.ELISA results showed that myocardial injury markers were significantly elevated in the LPS group compared with the control group,whereas the levels of myocardial injury markers were significantly reduced in the LPS+OSE group.Cardiac electrophysiological results showed that after intraperitoneal injection of LPS,the action potential(APD)and effective response period(ERP)of the rat heart were significantly shortened,and the average ERP and the levels of APD50 and APD90 of the rat heart in the LPS+OSE group were significantly increased compared with those of the LPS group.The Western results showed that after intraperitoneal injection of LPS,the expression of the protein level of Bax was significantly increased,while the expression of Bcl-2 protein was lower in the rat heart.elevated,while Bcl-2 protein was less expressed;compared with the LPS group,Bcl-2 expression in the myocardial tissue of rats in the LPS+OSE group was increased,and Bax protein level was decreased.Conclusion NEU1 inhibitor pretreatment can improve LPS-induced myocardial injury by attenuating inflammation and apoptosis,thus protecting cardiac function.