Expression of miR-146a and exploring the modulation mechanism of PAI-1 in the deep vein thrombosis

doi:10.3969/j.issn.1672-5301.2017.06.017

Abstract：Objective To detect the expression level of miRNA-146a and plasma PAI-1 in peripheral blood of the DVT patients.To explore the clinical significance in patients with DVT.Methods The specimens were divided into 3 groups:DVT cases group,the patient with the highly DVT risk factor but exclude clinical diagnose of DVT group,healthy control group,each group include 30 cases,selected peripheral blood plasma of each cases.The extraction and purification of miRNA from the plasma,using the methods of RT-qPCR to detect the relative expression level of miR-146a.The expression of PAI-1 in serum was determined by enzyme-linked immunosorbent assay,analysis the relationship between miRNA146a,PAI-1 and various clinical parameters in each group.Results (1)The relative expression of plasma miR-146a in DVT group decreased significantly than healthy control group (P＜0.01).The content of miR-146a in the high risk factors group after THA in comparison with healthy control group also decreased significantly (P＜0.01),whilethe relative expression level of miR-146a in gynecological tumor in the high-risk group was compared with the healthy control group increased significantly.(2)The level of PAI-1 in DVT group and high risk factors groupwas significantly increased than the healthy control group (P＜0.01).(3)Using Pearson correlation analysis method to analysis the relationship between the relative expression of plasma miR-146 concentration and the plasma level of PAI-1,the plasma miR-146a was negatively correlated with PAI-1 (r=-0.832,P＜0.01).Conclusion (1)The relative expression of miR-146a in DVT group was downregulated,and the expression was negatively correlated with the content of D-dimmer and PAI-1,which show that the miR-146a plays an important modulation role in the pathogenesis of DVT.(2)The plasma level of PAI-1 both in DVT group and high-risk factor group were increased,but increased more significantly in DVT group.It commanded that the important role of fibrinolysis in the formation of thrombosis.