Study on the correlation between PAPP-A polymorphism and coronary heart disease
ZHANG Qian
LI Xue-qin
WANG Xu
Abstract：Objective To investigate the polymorphism of PAPP-A gene in patients with coronary heart disease,and to explore the relationship between coronary heart disease and coronary heart disease.Methods A total of 100 patients with coronary heart disease in our hospital from February to January 2014 in our hospital were selected as the research subjects of this study,the experimental group was-2015.Select the same to our hospital for health examination of healthy people a total of 100 cases as the control group,first to collect blood samples for DNA extraction,and then use PCR gene amplification,the PCR products using ECOR V enzyme digestion and use PAPP-A amplification enzyme digestion products using agarose (2％) gel electrophoresis to identify PAPP-A gene type was observed PAPP-A gene polymorphism.Results PAPP-A gene type of a total of three,respectively was AA and CC homozygotes and AC type a heterozygous and AA type enzyme digestion performance 77 bp 1 a belt,CC type enzyme digestion is performance for a 107 bp,77 bp two bands,AC enzyme digestion is a 107 bp and 77 bp and 30 bp with three.The experimental group CC genotype was significantly higher than the control group,the experimental group,the frequency of C allele was higher than the control group (P＜0.05),the difference was statistically significant.Conclusion PAPP-A in patients with coronary heart disease (CHD) genotypes in occurred obvious change,mainly as an increase in the CC genotype,increase the frequency of C allele,so PAPP-A may has some relation with the incidence of CHD,provides a certain basis for clinical diagnosis and treatment.
Keywords：Coronary heart diseasePregnancy associated white AGene polymorphism
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 450-453 )