Effects of four insecticides on the activity of defence enzymes of Pleurotus pulmonarius and Auricularia polytricha

ZHANG Zhiying; QIU Qin; LIN Jinsheng; XU Ping; LI Huiping; HOU Lijuan; JIANG Ning; QU Shaoxuan; MA Lin; WANG Weixia; LI Fuhou

doi:10.16861/j.cnki.zggc.2025.0413

Abstract：To investigate the effects of insecticides on the activity of defense enzymes in edible fungi,Pleurotus pulmo-narius and Auricularia polytricha were used as test materials.The effects of four commonly used insecticides(difluben-zuron,dinotefuran,beta-cyhalothrin and chlorantraniliprole)on the activity of catalase(CAT),peroxidase(POD),superox-ide dismutase(SOD)and phenylalanine ammonia-lyase(PAL)in mycelia and fruiting bodies were determined by three methods,drug-containing plate culture,mixed drug in culture substrate and spraying before fruiting.The results showed that at the mycelial stage,only the SOD activity of P.pulmonarius and the PAL activity of A.polytricha treated with be-ta-cyhalothrin were significantly higher than the respective control.After application of beta-cypermethrin by mixing with the culture substrate,only the CAT activity in fruiting bodies of P.pulmonarius was significantly higher than that in the corresponding control,while the CAT,POD and SOD activity in fruiting bodies of A.polytricha were all significantly higher than those in the corresponding control.When the insecticide were sprayed before fruiting,no significant increase in defense enzyme activity was observed in insecticide-treated P.pulmonarius fruiting bodies compared to those in the corresponding control,while no significant decrease was found in A.polytricha under the same treatments.In conclusion,the application of the four insecticides led to varying degrees of changes in the defense enzyme activity of P.pulmonarius and A.polytricha in both mycelia and fruiting bodies.The variation patterns were mainly influenced by the differences in edible mushroom varieties,different growth stages,and the physicochemical properties of the insecticides such as their systemicity,persistence and permeability.