Extraction and antioxidant activity of total flavonoids and total phenols from vegetable jute leaves

HOU Wenhuan; LIAO Xiaofang; TANG Xingfu; ZHAO Yanhong

doi:10.16861/j.cnki.zggc.2025.0061

Abstract：To investigate the optimal ethanol volume fraction for extraction of total flavonoids and total phenols from veg-etable jute leaves and evaluate their antioxidant activity,leaves of Guimacai No.1 and Guimacai No.2 were used as ex-perimental materials.The effects of different ethanol volume fractions on the extraction yields of total flavonoids and total phenols were compared to determine the optimal extraction conditions.The antioxidant activities of the extracts were as-sessed by hydroxyl radical scavenging rate,DPPH radical scavenging rate,and ferrous ion chelating rate.The results showed that the optimal ethanol volume fraction for the extraction of total flavonoids and total phenols from vegetable jute leaves were 70%and 50%,respectively.The antioxidant capacity of total flavonoids and total phenols extracts from two vegetable jute cultivars enhanced with the increase of extract concentration.The half-maximal inhibitory concentra-tions(IC50)of total flavonoid extracts from Guimaicai 1 and Guimaicai 2 for scavenging hydroxyl radical were 0.738 and 0.708 mg·mL-1,and those of total phenol were 0.463 and 0.419 mg·mL-1,respectively.For scavenging DPPH radical the IC50 value of total flavonoids extracts from Guimacai No.1 and Guimacai No.2 were 1.151 and 1.153 mg·mL-1,and those of total phenol extracts were 0.662 and 0.639 mg·mL-1,respectively.The IC50 value of total flavonoids extracts from Gui-macai No.1 and Guimacai No.2 for chelating ferrous ions were 0.307 and 0.306 mg·mL-1,and those of total phenol extracts were 0.150 and 0.171 mg·mL-1,respectively.In conclusion,the optimal ethanol volume fractions for extracting total flavonoids and total phenols from vegetable jute leaves are 70%and 50%,respectively.Both total flavonoids and total phenols from edible jute leaves exhibit considerable antioxidant activity.