Quality evaluation of Ipomoer batatas Poir leaves in different collecting seasons based on HPLC fingerprint and principal component analysis
HAN Zhongyao
ZHANG Zhicheng
YU Shuangshuang
LIU Bangqin
LI Yan
LI Jiang
ZHANG Linsu
Abstract:The high performance liquid chromatography fingerprint of Ipomoer batatas Poir leaves in different collecting seasons was established,and the principal component analysis was carried out to provide reference for the quality control and quality evaluation of Ipomoer batatas Poir leaves in different collecting seasons.Taking 10 batches of Ipomoer bata-tas Poir leaves in different collecting seasons as research samples,the separation conditions of HPLC fingerprints of Ipo-moer batatas Poir leaves in different collecting seasons were explored and the samples were analyzed and detected.The fingerprints of Ipomoer batatas Poir leaves in different collecting seasons were established and the similarity was evaluat-ed,and the principal component analysis of Ipomoer batatas Poir leaves in different collecting seasons was carried out.The results showed that the established HPLC fingerprint method of Ipomoer batatas Poir leaves in different collecting seasons was stable and reliable.There were 10 common peaks in the fingerprint of Ipomoer batatas Poir leaves in differ-ent collecting seasons.After identification with reference substances,peak 3 was confirmed as chlorogenic acid absorp-tion peak.The quality of Ipomoer batatas Poir leaves in different collecting seasons from June to July fluctuated greatly,and there was a big difference in quality compared with the samples collected after August.The quality of Ipomoer bata-tas Poir leaves in different collecting seasons collected in August and later period tended to be stable.The similarity of 10 batches of Ipomoer batatas Poir leaves in different collecting seasons was 0.720-0.988.Two principal components were obtained by principal component analysis of Ipomoer batatas Poir leaves in different collecting seasons.And the cumula-tive contribution rate of the two principal components reached 86.849%.The results of this study laid a foundation for the quality control of Ipomoer batatas Poir leaves in different collecting seasons and the quality evaluation of related agricul-tural products developed.
Keywords:Ipomoer batatas Poir leavesHPLCFingerprintSimilarityPrincipal component analysisChlorogenic acid
Publication Date:2026-02-05
Online Publishing Date:2026-03-10(First online date of this platform, not the publication date of the document)
Pages:8( 182-189 )
