Optimization of potato anther culture techniques

ZHU Xu; WEI Jia; LI Nan; MU Shujing; GUAN Kexing; YU Hongzhu; HE Hongxia; LI Yidan

doi:10.16861/j.cnki.zggc.2025.0419

Abstract：Anther cultivation of potato diploids is a critical technology for obtaining diploid plants and accelerating ploidy breeding.However,challenges such as low induction frequency and difficulties in seedling differentiation have limited its widespread application.This study aimed to establish an efficient pollen culture system to address these bottlenecks.Us-ing pollen from tetraploid potato variety Neishu No.7 as material,the culture medium formula was optimized to identify components suitable for pollen tissue induction and plant differentiation in this variety.The results showed that the medi-um MS+30 g·L-1 sucrose+7 g·L-1 agar+1 mg·L-1 nicotinate(NAA)+1 mg·L-12,4-dichlorophenoxyacetic acid(2,4-D)+0.5 mg·L-1 cytokinin(KT)demonstrated superior efficacy in inducing potato pollen callus formation.The medium MS+30 g·L-1 sucrose+7 g·L-1 agar+5 g·L-1 activated charcoal(AC)+0.5 mg·L-1 indoleacetic acid(IAA)+2 mg·L-1 zeatin ZT+2 mg·L-16-benzylaminoguanine(6-BA)exhibited better differentiation performance.This system successfully induced diploid plants in D126 potato,preliminarily demonstrating broad adaptability potential.The study successfully established an efficient pollen culture system for Neishu No.7,significantly improving cultivation efficiency while showing promise for applications in other varieties,which lay a foundation for diploid potato breeding.