Effects of light quality and hormone combination on in vitro culture of stem segments from male sterile watermelon
LI Hailun
LI Xiaohui
KANG Liyun
ZHAO Weixing
CHANG Gaozheng
WANG Huiying
GAO Ningning
Abstract：Using stem segments with buds from male sterile watermelon plants as explants,this study optimized the ex-plant disinfection method and systematically investigated the effects of different light qualities on adventitious bud induc-tion,proliferation,and rooting,aiming to establish an efficient tissue culture and rapid propagation system for watermelon stem segments.The results showed that disinfecting with 75%ethanol for 30 seconds followed by treatment with 0.1%mercuric chloride for 8 min was the optimal disinfection method,effectively reducing the contaimination rate and achiev-ing a survival rate of 85.56%.Under the light quality condition of 4R1B,combined with the optimal induction medium(MS+2 mg·L-16-BA+0.1 mg·L-1 NAA),proliferation medium(MS+0.2 mg·L-1 KT+10%coconut water),and rooting me-dium(1/2MS+0.1 mg·L-1 IAA+10%coconut water),the adventitious induction rate increased to 95.00%,the prolifera-tion coefficient reached 4.80,and the rooting rate reached 100.00%.This study effectively optimized the tissue culture and rapid propagation system for watermelon,providing technical support for the efficient rapid propagation and long-term perservation of high-quality and scarce watermelon germplasm resources,and laying a foundation for the sus-tainable utilizaiton of watermelon germplasm resources.
Keywords：WatermelonStem segmentTissue cultureSterilizationDifferent light qualitiesRapid propagation
Publication Date：2026-02-05
Online Publishing Date：2026-03-10(First online date of this platform, not the publication date of the document)
Pages：12( 20-31 )