Analysis of endophytic bacteria diversity and growth-promoting func-tions in melon(Cucumis melo L.)seeds
SUN Beibei
ZHOU Yu
WANG Lijun
XUAN Zhengying
Abstract：To identify endophytes in melon(Cucumis melo L.)seeds and verify their plant growth-promoting functions,melon seeds were used as the experimental materials.Endophytes were isolated using the dilution plating method,and their diversity was analyzed based on 16S rRNA gene sequencing.The isolated endophytes were evaluated for their abili-ties in phosphate solubilization,nitrogen fixation,potassium solubilization,production of indole-3-acetic acid(IAA),sid-erophore production,and cellulose decomposition using various growth-promoting media.The results showed that 39 bac-terial strains were successfully isolated from the melon seeds,belonging to 7 species across 3 genera(Bacillus,Escherich-ia,Shigella),with Bacillus being the dominant genus.All species exhibited nitrogen fixation and IAA production capabili-ties,while 5 species belonging to the Bacillus and Escherichia genera demonstrated siderophores production.In conclu-sion,the melon seeds harbored diverse endophytes,and all endophytes possess growth-promoting functions.This study re-veals the diversity of endophyte in melon seeds and provides valuable germplasm resources for the development of envi-ronmentally friendly biofertilizers.
Keywords：Melon seedsEndophytesDiversityPlant growth promotion
Publication Date：2026-01-05
Online Publishing Date：2026-04-02(First online date of this platform, not the publication date of the document)
Pages：7( 109-115 )