Identification of the Hsp gene family in Pleurotus ostreatus and expres-sion analysis under heat stress
REN Xinhui
GUO Lifeng
WANG Jingyi
HOU Ludan
MENG Junlong
CHANG Mingchang
WANG Shurong
Abstract：Heat shock protein(Hsp)is a class of molecular chaperones produced by organisms under heat stress or other environmental stimuli and plays important roles in abiotic stress responses.In this study,the Hsp gene family in the Pleu-rotus ostreatus CCMSSCC00389 strain was identified by bioinformatics,and its expression patterns under heat stress were analyzed.The results showed that a total of 27 PoHsps were identified in the P.ostreatus genome and were unevenly distributed across 11 chromosomes.The analysis results of cis-acting elements in gene promoters show that hormone-re-sponsive cis-acting elements account for the largest proportion.Further investigation revealed that under heat stress at dif-ferent temperatures,the expression patterns of PoHsps could be broadly classified into five categories:PoHsp19 expres-sion gradually decreased with increasing temperature;PoHsp7 and PoHsp24 expression did not significantly change un-der different heat stress conditions;and the expression patterns of PoHsp2,PoHsp4,PoHsp6,and PoHsp15 presented sim-ilar expression patterns,all of which initially decreased but then increased;however,the other PoHsps gradually increased with increasing stress temperature.These findings suggest that PoHsps may have functional differences in response to var-ious stress conditions.This study provides a foundation for further functional characterization of the Hsp gene family in P.ostreatus under abiotic stress.
Keywords：Pleurotus ostreatusHsp gene familyHeat stressBioinformatics analysis
Publication Date：2026-01-05
Online Publishing Date：2026-04-02(First online date of this platform, not the publication date of the document)
Pages：13( 26-38 )