Experiment of the cultivation of Naematelia aurantialba by different inoc-ulation methods of Jiner spores and Stereum hirsutum agar disks
WANG Nengshuang
MA Qingfang
ZHANG Piqi
MA Shiyu
MA Yinpeng
Abstract：To identify the optimal inoculation method for cultivating Naematelia aurantialba using Jiner spores and Stere-um hirsutum,eight different inoculation strategies involving Jiner spores and S.hirsutum were tested.Initially,the method that resulted in the earliest fruiting and better quality of N.aurantialba fruiting bodies was identified.Subsequently,a con-trolled experiment was conducted by setting two conditions in this method—inoculum volume of Jiner spores and interval days between the inoculation of the two fungi—as variables.By comparing indicators including mycelial growth rate,time to full bottle colonization,time to primordia formation,fruiting body traits,and biological efficiency,the inoculation method that resulted in the earliest fruiting and superior fruiting body traits was ultimately determined.The results showed that method A—first inoculating one block of S.hirsutum(6 mm in diameter),followed 8 days later by 1 mL of Jiner spore suspension(1×104 mL-1)—resulted in the best mycelial growth and the earliest primordium formation.Further optimization of method A revealed that the optimal inoculation method A5—first inoculating one block of S.hirsutum,fol-lowed 8 days later by 0.5 mL of Jiner spore suspension—achieved the best mycelial growth,early primordium formation(30 days),and the highest biological efficiency(48.4％).The results of this study provides technical support for mixed in-oculation of Jiner spore suspensions and S.hirsutum cultures in the cultivation of N.aurantialba.
Keywords：Naematelia aurantialbaSporeStereum hirsutumInoculation methodAgronomic character
Publication Date：2025-12-05
Online Publishing Date：2026-01-05(First online date of this platform, not the publication date of the document)
Pages：6( 141-146 )