Establishment of rapid genomic DNA extraction method from single dry celery seed
WU Feng
LI Kexin
WANG Wutai
HUA Deping
GAO Guoxun
Abstract：Genomic DNA extracted directly from plant seeds could be used for molecular identification of seed purity and specific genotypes.There is no report on efficient direct extraction methods for genomic DNA from single dry celery seed until now.In this study,the single dried celery seed was subjected to extract genome DNA by alkaline lysis method,which was utilized as the template for PCR(polymerase chain reaction)amplification withActin-specific primers to verify whether the extracted genomic DNA meets the requirements for molecular detection.The results showed that clear specific bands were obtained by PCR amplification,demonstrating that the genomic DNA directly extracted from single dry celery seed was suitable for PCR amplification.To assess DNA stability,PCR amplification was conducted using the genomic DNA stored at 4 ℃ for 14 days,and successful amplification was confirmed by the presence of expected bands in agarose gels.This study established a rapid,simple and highly efficient method for extracting genomic DNA from individual dried celery seed.The extracted genome DNA was suitable for PCR amplification and exhibited good stability.It could be used for MAS of celery seed purity and specific genotypes.
Keywords：CeleryGenomic DNA extractionSingle seedMolecular marker assisted breeding
Publication Date：2025-12-05
Online Publishing Date：2026-01-05(First online date of this platform, not the publication date of the document)
Pages：6( 71-76 )