Function identification of tomato AMR1 gene in ascorbic acid synthesis and metabolism
LI Yan
SUN Xueyu
ZHANG Yuyang
WANG Mengyang
ZHAO Hongxing
YANG Fan
WU Liheng
Abstract：In order to deeply investigate the specific regulatory mechanism of SlAMR1,an ascorbic acid(AsA)synthesis regulatory factor in tomato,the homologus gene SlAMR1 in tomato was screened using the Arabidopsis thaliana AMR1 gene as a probe.Overexpression and RNA interference(RNAi)expression vectors for the SlAMR1 gene were constructed and introduced into the tomato line Ailsa Craig(AC)via Agrobacterium-miediated genetic transformation.The expression levels of theAMR1 gene,key genes of AsA synthesis pathway,and the corresponding AsA content in the transgenic mate-rials were determined.The results showed that the content of AsA in the young leaves of OE6-8 and OE12-6 lines decreased by 92.66％and 95.41％compared with the control,respectively.In contrast,the AsA content in the young leaves of RNAi2-4 and RNAi13-11 increased by 26.86％and 56.49％,respectively.Evolutionary analysis in different species showed that SlAMR1 and AtAMR1 protein had relatively high homology,and SlAMR1 domain analysis also showed that SlAMR1 and AtAMR1 protein had the same domain at the N-terminal and C-terminal regions.This study pre-liminarily revealed the role of SlAMR1 gene in the regulation of AsA biosynthesis and metabolism in tomato.
Keywords：TomatoAscorbic acidRegulatory factorGenetic transformation
Publication Date：2025-12-05
Online Publishing Date：2026-01-05(First online date of this platform, not the publication date of the document)
Pages：9( 36-44 )