Comparative study on the applicability of three DNA extraction methods from melon leaves for KASP marker detection system
LI Wulin
MA Jiaxin
HE Yuhua
TANG Lingli
XU Yongyang
HUANG Xiang
ZHAO Guangwei
Abstract：To investigate DNA extraction methods from melon leaves suitable for Kompetitive Allele-Specific PCR(KASP)marker detection system,this study used melon parents B551,B22 and their F1 hybrid(B551×B22)as materi-als.Three DNA extraction methods including alkaline lysis,CTAB and a commercial magnetic bead-based kit-were em-ployed to extract DNA from melon leaves.The effects of these three DNA extraction mehods on the KASP marker detec-tion system were analyzed.DNA quality assessment results showed that the commercial kit method effectively removed impurities such as proteins,polyphenols,and polysaccharides from melon leaves,achieving a KASP detection accuracy of 100％.The CTAB method achieved a detection accuracy of 97.2％,while the alkaline lysis method achieved a detection of 69.4％.In conclusion,the commercial magnetic bead-based kit is the most suitable for high-throughput KASP detection,the CTAB method can be used for small-scale detection,and the alkaline lysis method is not suitable for DNA extraction from melon leaves in KASP marker detection systems.
Keywords：Cucumis meloKASP genotypingDNA extraction method
Publication Date：2025-12-05
Online Publishing Date：2026-01-05(First online date of this platform, not the publication date of the document)
Pages：7( 30-35,44 )