Optimizing the fermentation medium of biocontrol Streptomyces PBSH9 against potato common scab using response surface methodology
ZHANG Min
WAN Zhongyi
FANG Wei
ZHANG Yani
LIU Fang
KE Shaoyong
ZHANG Zhicheng
DONG Yongyi
ZHANG Xiaoyu
Abstract：Potato common scab is a significant disease that restricts the development of the potato industry.This study screened the biocontrol strain PBSH9,and its metabolites showed significant inhibitory effects on the pathogen,achieving a field control efficacy of 73.97％.To identify the active compounds,the secondary metabolites of strain PBSH9 were iso-lated and purified,successfully yielding the active substance.To enhance the yield of this active substance,response sur-face methodology(RSM)was employed to optimize its culture medium.Based on single-factor experiments and Plack-ett-Burman tests,combined with the steepest ascent method and Box-Behnken design response surface methodology to fit the nonlinear equation between significant factors and the yield of active compound,the optimal fermentation medium for-mula was obtained.The optimized formula was 4.50 g·L-1 glucose,50.00 g·L-1 soluble starch,15.00 g·L-1 corn flour,21.08 g·L-1 soybean meal,2.00 g·L-1 peptone,0.50 g·L-1(NH4)2SO4,0.60 g·L-1 MgSO4,and 0.04 g·L-1 KH2PO4.The mod-el-predicted yield of active compounds was 199.92 μg·mL1,while the actual value was 184.35 μg·mL-1,which was 5.83 times higher than the yield of compounds in the basic culture medium.This study provides crucial technical sup-port for the development of efficient biopesticide products against potato common scab.
Keywords：Potato common scabBiocontrol StreptomycesResponse surface methodologyBioactive metabolitesMedium
Publication Date：2025-11-05
Online Publishing Date：2025-12-04(First online date of this platform, not the publication date of the document)
Pages：12( 65-76 )